Fågel på gren
Parningen sker oftast på en gren eller på marken. De flesta andfåglar parar sig dock i vattnet och seglare parar sig i luften. Parningen kan ske mycket snabbt, ibland tar det under en halv sekund. Ultrapure molecular biology grade agarose pre-coated with GelGreen® Nucleic Acid Gel Stain for convenient and safer preparation of precast gels. Request the Certificate of Analysis. Most of our products are stable at room temperature for many days, so in all likelihood the product will still work just fine.
To be on the safe side, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples. If this occurs, the GelGreen will no longer work for gel staining. The main difference between GelRed® and GelGreen® is their fluorescence excitation and emission wavelengths. GelRed® has red fluorescence, similar to ethidium bromide.
Both dyes are compatible with standard UV transilluminators. GelGreen® is also compatible with blue light transilluminators, which allow users to avoid exposing themselves and their DNA samples to ultraviolet radiation. GelRed® and GelGreen® have higher sensitivity for double stranded nucleic acids compared to single stranded nucleic acids, but GelRed® is more sensitive for staining single stranded nucleic acids than GelGreen®.
GelRed® is about twice as sensitive for double stranded nucleic acids compared to single-stranded nucleic acids, and about five times more sensitive than GelGreen® for staining single stranded nucleic acids. For more information about these products, please visit our DNA stains technology page. View the GelRed® Product Line. View the GelGreen® Product Line. The water formulation is a newer and improved product compared to the stock in DMSO.
We recommend using dyes in water to avoid the potential hazards of handling DMSO, which can be absorbed through the skin. We continue to offer dyes in DMSO because some users do not wish to alter their established laboratory protocols. Based on internal testing, both formulations perform similarly. View more FAQs. Many customers use GelRed® or GelGreen® precast gels for convenience. However, because GelRed® and GelGreen® are high affinity dyes designed to be larger dyes to improve their safety, they can affect the migration of DNA in precast gels.
If you see band migration shifts or smearing and smiling, try reducing the amount of DNA loaded. For samples of unknown concentration, try loading one half or one third of the usual amount of DNA. This usually solves band migration problems.
Tälj små fåglar till granen
To avoid any interference the dye may have on DNA migration, we recommend using the post-staining protocol. If your application requires loading more than the recommended amount of DNA, use the post-staining protocol. While we recommend post-staining gels for 30 minutes, you may be able see bands in as little as five minutes, depending on how much DNA is present. Post-staining solutions can be reused. Wishlist updated!
View wishlist. With GelGreen® Agarose, there is no need to handle concentrated fluorescent dye while preparing your gel, for greater convenience and safety. This agarose has excellent performance for analytical or preparative nucleic acid electrophoresis and blotting. It is suitable for preparing 0. GelGreen® Agarose also gives excellent results at percentages between 0.
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GelGreen® is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye specifically designed for gel staining. GelGreen® has UV absorption between nm and nm and a strong absorption peak centered around nm. With blue light illuminators, researchers can avoid exposure to UV irradiation for themselves and their DNA samples, for a safer work environment and higher cloning efficiency. Gel staining with GelGreen® is compatible with downstream applications such as sequencing and cloning.
Test results confirm that the dye is impenetrable to both latex gloves and cell membranes. The dye is non-cytotoxic and non-mutagenic at concentrations well above the working concentrations used in gel staining. Using GelGreen® Agarose further minimizes risk by avoiding the need to handle concentrated dye solution. Skip to content. Product Catalog.
DNA Quantitation Kits. RNA Quantitation Kits.